p63 Recombinant Antibody [SC06-31] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610429
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clone number: | SC06-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Immunogen: | Recombinant protein within human p63 aa 1-250. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cell) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:5,000 1:1,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: Q9H3D4 Human | O88898 Mouse | Q9JJP6 Rat |
| Alternative names: | AIS Amplified in squamous cell carcinoma B(p51A) B(p51B) Chronic ulcerative stomatitis protein CUSP DN p63 alpha 1 DNp63 EEC3 id:ibd3516 Keratinocyte transcription factor Keratinocyte transcription factor KET KET LMS MGC115972 MGC192897 NBP OFC8 OTTHUMP00000209732 OTTHUMP00000209733 OTTHUMP00000209734 OTTHUMP00000209735 OTTHUMP00000209737 OTTHUMP00000209738 OTTHUMP00000209739 OTTHUMP00000209740 OTTHUMP00000209741 OTTHUMP00000209742 OTTHUMP00000209743 OTTHUMP00000209744 p40 p51 P51/P63 p53-related protein p63 p53CP p63 P63_HUMAN p73H p73L RHS SHFM4 TAp63alpha TP53CP TP53L TP63 TP73L Transformation related protein 63 Transformation-related protein 63 Trp53rp1 Trp63 Tumor protein 63 Tumor protein p53-competing protein Tumor protein p53-like Tumor protein p63 Tumor protein p63 deltaN isoform delta Tumor protein p73 Tumor protein p73-like |
Images
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Fig1:
Western blot analysis of p63 on different lysates with Mouse anti-p63 antibody (HA610429) at 1/5,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Negative control: MCF7 (PMID: 17446929). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610429, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 77 kDa Observed band size: 63 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: A431 (Human epidermoid carcinoma skin squamous cell) / MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610429, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Low expression: MCF7 (PMID: 26704768). |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610429, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610429, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610429, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610429, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.