TTF1 Recombinant Antibody [JE61-73] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610433
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P |
| Clone number: | JE61-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Immunogen: | Recombinant protein within human TTF1 aa 272-371/371. |
| Positive control: | TT, Human Lung adenocarcinoma, Human Lung, Mouse Lung, Rat Lung. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Cell IHC-P |
1:500 1:500 |
| Uniprot #: | SwissProt: P43699 Human | P50220 Mouse | P23441 Rat |
| Alternative names: | AV026640 BCH Benign chorea BHC Homeobox protein NK 2 homolog A Homeobox protein NK-2 homolog A Homeobox protein Nkx 2.1 Homeobox protein Nkx-2.1 Homeobox protein Nkx2.1 NK 2 NK 2 homolog A NK2 NK2 homeobox 1 NK2, drosophila, homolog of, A NK2.1, mouse, homolog of Nkx 2 1 NKX 2.1 NKX 2A NKX2 1 Nkx2-1 NKX2.1 NKX21_HUMAN NKX2A T EBP T/EBP TEBP Thyroid nuclear factor 1 Thyroid nuclear factor Thyroid specific enhancer binding protein Thyroid transcription factor 1 Tin man Tinman TITF 1 TITF1 TTF 1 TTF-1 TTF1 |
Images
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Fig1:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: TT (Human medullary thyroid carcinoma cell) / HeLa (Human medullary thyroid carcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610433, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). Negative control: HeLa (PMID: 16260629). |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung adenocarcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610433, 1/500, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610433, 1/500, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610433, 1/500, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610433, 1/500, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.