Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric) - BSA and Azide free
cat.: HA610440
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, FC(Intra) |
| Clone number: | JM100-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Immunogen: | Recombinant protein within Human Paravalbumin aa 1-110 / 110. |
| Positive control: | RPMI8226 (Human multiple myeloma cell) cell lysate, Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysates. |
| Subcellular location: | Axon, cytoplasm, synapse. |
| Recommended Dilutions:
WB IHC-Fr IHC-P FC(Intra) |
1:5,000 1:500 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P20472 Human | P32848 Mouse | P02625 Rat |
| Alternative names: | D22S749 MGC116759 Parvalbumin alpha PRVA_HUMAN PVALB |
Images
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Fig1:
Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (HA610440) at 1/5,000 dilution. Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate Lane 3: Mouse skeletal muscle tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
|
Fig2:
Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (HA610440) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa |
|
Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610440, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610440, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610440, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1146, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610440, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1146, 20 minutes at room temperature. |
|
Fig7:
Application: Flow Cytometry (Intra)
Species: Human Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA610440(1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 15 minutes at room temperature. Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.