SOX9 Recombinant Antibody [SN74-09] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610441
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clone number: | SN74-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Immunogen: | Recombinant protein within human SOX9 aa 140-340. |
| Positive control: | Human Colon, Mouse Colon, Rat Colon. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P |
1:2,000 |
| Uniprot #: | SwissProt: P48436 Human | Q04887 Mouse | F1LYL9 Rat |
| Alternative names: | campomelic dysplasia autosomal sex reversal CMD 1 CMD1 CMPD 1 CMPD1 SOX 9 Sox9 SOX9_HUMAN SRA 1 SRA1 SRXX2 SRXY10 SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal SRY (sex determining region Y) box 9 SRY (sex determining region Y)-box 9 SRY (sex-determining region Y)-box 9 protein SRY related HMG box gene 9 Transcription factor SOX 9 Transcription factor SOX-9 transcription factor SOX9 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610441, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610441, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610441, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.