Beta III Tubulin Recombinant Antibody [PSH15-32] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610444
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, IF-Tissue |
| Clone number: | PSH15-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Immunogen: | Synthetic peptide within mouse Beta III Tubulin aa 401-450. |
| Positive control: | Neuro-2a, Human Cerebellum, Mouse Cerebellum, Rat Cerebellum. |
| Subcellular location: | Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium. |
| Recommended Dilutions:
IF-Cell IHC-P IF-Tissue |
1:50 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q13509 Human | Q9ERD7 Mouse | Q4QRB4 Rat |
| Alternative names: | beta 3 tubulin beta 4 beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III tuj 1 tuj1 |
Images
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Fig1:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Neuro-2a (Mouse brain neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610444, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610444, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610444, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610444, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610444, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610444, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig7:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610444, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.