Parkin Recombinant Antibody [PSH08-11] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
cat.: HA610448
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue, FC(Intra) |
| Clone number: | PSH08-11 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Immunogen: | Recombinant protein within human Parkin aa 1-200. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse. |
| Uniprot #: | SwissProt: O60260 Human | Q9WVS6 Mouse | Q9JK66 Rat |
| Alternative names: | AR JP E3 ubiquitin ligase E3 ubiquitin protein ligase parkin E3 ubiquitin-protein ligase parkin FRA6E LPRS 2 LPRS2 PARK 2 Park2 Parkin 2 Parkinson disease (autosomal recessive juvenile) 2 Parkinson disease (autosomal recessive, juvenile) 2, parkin Parkinson disease protein 2 Parkinson juvenile disease protein 2 Parkinson protein 2 E3 ubiquitin protein ligase Parkinson protein 2, E3 ubiquitin protein ligase (parkin) PDJ PRKN 2 PRKN PRKN2 PRKN2_HUMAN Ubiquitin E3 ligase PRKN |
Images
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Fig1:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Western blot analysis of Parkin on different lysates with Guinea pig anti-Parkin antibody (HA610448) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: COS-1 (African green monkey kidney fibroblast) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Pig brain tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610448, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
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Fig2:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610448, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 45 hours at room temperature. Counterstain: Beta tubulin (HA601187, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610448, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1021, 30 minutes at room temperature. |
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Fig4:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610448, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1021, 30 minutes at room temperature. |
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Fig5:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610448, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
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Fig6:
This data was developed using HA610448, the same antibody clone in a different buffer formulation. Application: Flow Cytometry (Intra) Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA610448 (1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 15 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.