PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610458
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clone number: | SN07-21 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Immunogen: | Synthetic peptide within Human PARK7 aa 11-60 / 189. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus. |
| Uniprot #: | SwissProt: Q99497 Human | Q99LX0 Mouse | O88767 Rat |
| Alternative names: | CAP1 DJ-1 DJ1 DJ1 protein Epididymis secretory sperm binding protein Li 67p FLJ27376 FLJ34360 FLJ92274 HEL S 67p Oncogene DJ1 OTTHUMP00000001348 OTTHUMP00000001349 OTTHUMP00000001350 OTTHUMP00000001351 PARK7 PARK7_HUMAN Parkinson disease (autosomal recessive, early onset) 7 Parkinson disease protein 7 Parkinson protein 7 Protein DJ-1 SP22 |
Images
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Fig1:
This data was developed using HA610458, the same antibody clone in a different buffer formulation. Western blot analysis of PARK7/DJ1 on different lysates with Rat anti-PARK7/DJ1 antibody (HA610458) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610458, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 20 kDa |
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Fig2:
This data was developed using HA610458, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610458, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1123, 20 minutes at room temperature. |
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Fig3:
This data was developed using HA610458, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610458, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1123, 20 minutes at room temperature. |
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Fig4:
This data was developed using HA610458, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610458, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1123, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.