Histone H3 Recombinant Antibody [6-A7-R] - Chicken IgY (Chimeric) - BSA and Azide free
cat.: HA610463
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clone number: | 6-A7-R |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Immunogen: | Synthetic peptide within Human Histone H3 aa 1-50 / 136. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate. |
| Subcellular location: | Nucleus. |
| Uniprot #: | SwissProt: P68431 human | P84243 human | Q16695 human | Q6NXT2 human | Q71DI3 human | P68433 mouse | P84228 mouse | Q6LED0 rat |
| Alternative names: | H3 histone family member E pseudogene H3 histone family, member A H3/A H31_HUMAN H3F3 H3FA Hist1h3a HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST3H3 histone 1, H3a Histone cluster 1, H3a Histone H3 3 pseudogene Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l |
Images
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Fig1:
This data was developed using HA610463, the same antibody clone in a different buffer formulation. Western blot analysis of Histone H3 on different lysates with Chicken anti-Histone H3 antibody (HA610463) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610463, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Chicken IgY IgG-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 15 kDa Observed band size: 15 kDa |
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Fig2:
This data was developed using HA610463, the same antibody clone in a different buffer formulation. Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610463, 1/200, 1 hour at room temperature. Secondary antibody: HA1146, 20 minutes at room temperature. |
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Fig3:
This data was developed using HA610463, the same antibody clone in a different buffer formulation. Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610463, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.