Biotin Conjugated Human PARK7 Recombinant Mouse Monoclonal Antibody [PSH15-68]
cat.: HA620002B
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH15-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human PARK7 aa 1-189 (HA211158). |
| Positive control: | Recombinant Human PARK7 protein (HA211158). |
| Subcellular location: | Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH15-67] to Human PARK7 antibody (Capture) (HA620001) and Recombinant Human PARK7 protein (HA211158) as the standard. The reference range value is 39.1-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q99497 Human |
| Alternative names: | CAP1 DJ-1 DJ1 DJ1 protein Epididymis secretory sperm binding protein Li 67p FLJ27376 FLJ34360 FLJ92274 HEL S 67p Oncogene DJ1 OTTHUMP00000001348 OTTHUMP00000001349 OTTHUMP00000001350 OTTHUMP00000001351 PARK7 PARK7_HUMAN Parkinson disease (autosomal recessive, early onset) 7 Parkinson disease protein 7 Parkinson protein 7 Protein DJ-1 SP22 |
Images
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Fig1:
Sandwich ELISA analysis of Human PARK7 matched pair antibodies Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA620001) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PARK7 protein (HA211158) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA620002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PARK7 in 0.125 mg/ml HL-60 lysate samples. Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] The concentrations of PARK7 were measured in duplicates, interpolated from the PARK7 standard curve and corrected for sample dilution. Undiluted samples are 0.125 mg/ml HL-60 lysate . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PARK7 concentration was determined to be 64.8 ng/ml in HL-60 lysate. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.