HA tag Nanoselector Agarose
cat.: HA710007
| Product Type: | Alpaca monoclonal, Nanoselector |
|---|---|
| Host: | Alpaca |
| Applications: | IP, Co-IP, Mass Spectrometry, ChIP |
| Clonality: | Recombinant |
| Form: | Agarose slurry |
| Storage condition: | Store at +4C. Do not freeze. |
| Storage buffer: | 20% EtOH |
| Target: | HA tag |
| Synonyms: | The HA Nanoselector recognizes the HA-tag sequence motif YPYDVPDYA at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Property: | Bead size: ~ 90 um |
| Affinity: | Affinity: 1.3nM; 10 µL slurry bind about 20 µg of recombinant GFP-HA tag. |
| Elution: | SDS sample buffer, 0.2 M glycine pH 2.5, 40 µM YPYDVPDYA peptide. For optimal performance, please consider on-bead assays like on-bead digestion for MS analysis. |
Images
|
Fig1:
Immunoprecipitation of N-HA protein With HA tag Nanoselector Agarose. Lane 1:Input Lane 2: Flow-through Lane 3: PH=3 Elution Lane 4: PH=2 Elution Lane 5: Eluted Beads |
|
Fig2:
Immunoprecipitation of C-HA protein With HA tag Nanoselector Agarose. Lane 1:Input Lane 2: Flow-through Lane 3: PH=3 Elution Lane 4: PH=2 Elution Lane 5: Eluted Beads |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.