Mouse IgG kappa, AlpSdAbs® VHH Alpaca Monoclonal Antibody
cat.: HA710008
Product Type: Alpaca monoclonal VHH domain of alpaca IgG2b/2c, secondary antibodies
Species reactivity: Mouse
Applications: WB, ELISA
Clonality: Monoclonal
Form: Liquid
Storage condition: Store at –20 ℃(Avoid freeze / thaw cycles), Stable for 12 months at -20℃
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Isotype: VHH domain of alpaca IgG2b/2c
Immunogen: Fab region of Mouse IgG
Recommended Dilutions:
  WB
  ELISA

1:5,000-1:20,000
1:5,000-1:20,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710008_1.jpeg Fig1: ELISA of specificity for different species of IgG-Anti-Mouse IgG kappa, AlpSdAbs® VHH

ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG kappa, AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG as the secondary antibody.
HA710008_2.jpeg Fig2: A titer ELISA of mouse IgG-Anti-Mouse IgG kappa, AlpSdAbs® VHH

A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG(kappa light chain). 1:5000 dilution of Anti-Mouse IgG kappa, AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.