Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c, secondary antibodies |
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Species reactivity: | Mouse |
Applications: | WB, ELISA |
Clonality: | Monoclonal |
Form: | Liquid |
Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles), Stable for 12 months at -20℃ |
Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 |
Concentration: | 1ug/ul |
Purification: | Recombinant Expression and Affinity purified |
Isotype: | VHH domain of alpaca IgG2b/2c |
Immunogen: | Fab region of Mouse IgG |
Recommended Dilutions:
WB ELISA |
1:5,000-1:20,000 1:5,000-1:20,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Fig1:
ELISA of specificity for different species of IgG-Anti-Mouse IgG kappa, AlpSdAbs® VHH ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG kappa, AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG as the secondary antibody. |
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Fig2:
A titer ELISA of mouse IgG-Anti-Mouse IgG kappa, AlpSdAbs® VHH A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG(kappa light chain). 1:5000 dilution of Anti-Mouse IgG kappa, AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG as the secondary antibody. |