HRP Conjugated Camel IgG(H+L), AlpHcAbs® Alpaca Monoclonal Antibody
cat.: HA710012
| Product Type: | Alpaca monoclonal Goat IgG, secondary antibodies |
|---|---|
| Applications: | WB, IHC, ELISA |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20℃ |
| Storage buffer: | 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol |
| Concentration: | 1ug/ul |
| Purification: | Affinity purified |
| Isotype: | Goat IgG |
| Immunogen: | Camelus dromedarius immunoglobulins |
| Recommended Dilutions:
WB ELISA IHC IHC-Paraffin |
1:5000-1:50000 1:10000-1:50000 1:200 - 1:5000 1:200-1:5000 Almost it can be used for VHH that come from Camel. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.