Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody Alpaca pacous Monoclonal
cat.: HA710022
Product Type: Alpaca monoclonal, secondary antibodies
Applications: WB, ELISA
Clonality: Monoclonal
Storage condition: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Immunogen: Recombinant Fc region of goat IgG
Recommended Dilutions:
  ELISA
  WB

1:5000-1:20000
1:5000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710022_1.jpg Fig1: A titer ELISA of Goat IgG-Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody
A titer ELISA of Goat IgG. The plate was coated with different amounts of Goat IgG. 1:5000 dilution of Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG(HA710046) as the secondary antibody.
HA710022_2.jpg Fig2: ELISA of specificity for different species of IgG-Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG(HA710046) as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.