Product Type: | Alpaca monoclonal, secondary antibodies |
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Applications: | WB, ELISA |
Clonality: | Monoclonal |
Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C |
Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
Concentration: | 1ug/ul |
Purification: | Recombinant Expression and Affinity purified |
Isotype: | VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc |
Immunogen: | Recombinant Fc region of goat IgG |
Recommended Dilutions:
ELISA WB |
1:5000-1:20000 1:5000-1:20000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Fig1:
A titer ELISA of Goat IgG-Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody A titer ELISA of Goat IgG. The plate was coated with different amounts of Goat IgG. 1:5000 dilution of Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG(HA710046) as the secondary antibody. |
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Fig2:
ELISA of specificity for different species of IgG-Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Goat IgG(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG(HA710046) as the secondary antibody. |