Product Type: | Alpaca monoclonal, secondary antibodies |
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Applications: | WB, ELISA |
Clonality: | Monoclonal |
Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C |
Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
Concentration: | 1ug/ul |
Purification: | Recombinant Expression and Affinity purified |
Isotype: | VHH domain of alpaca IgG2b/2c fused to goat IgG Fc |
Immunogen: | Recombinant Fc region of chicken IgY |
Recommended Dilutions:
ELISA WB |
1:10000-1:50000 1:10000-1:50000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Fig1:
ELISA of specificity for different species of IgG-Anti-Chicken IgY, AlpHcAbs® Goat antibody ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Chicken IgY, AlpHcAbs® Goat antibody was used as the primary antibody. An HRP conjugated anti-Goat IgG(HA710020) as the secondary antibody. |
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Fig2:
A titer ELISA of chicken IgY-Anti-Chicken IgY, AlpHcAbs® Goat antibody A titer ELISA of chicken IgY. The plate was coated with different amounts of chicken IgY. 1:10000 dilution of Anti-Chicken IgY, AlpHcAbs® Goat antibody was used as the primary antibody. An HRP conjugated anti-Goat IgG(HA710020) as the secondary antibody. |