Human kappa, AlpHcAbs® Goat antibody Alpaca pacous Monoclonal
cat.: HA710063
| Product Type: | Alpaca monoclonal, secondary antibodies |
|---|---|
| Applications: | WB, ICC/IF, ELISA, IP, Flow Cyt |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C |
| Storage buffer: | 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) |
| Immunogen: | Fab region of human IgG |
| Recommended Dilutions:
ELISA WB ICC/IF IP Flow Cyt |
1:5000-1:20000 1:5000-1:20000 1:200-1:1000 1-2ug/sample 1µg for 106 cells Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.