DYKDDDDK tag, AlpHcAbs® Mouse antibody Alpaca Monoclonal
cat.: HA710084
| Product Type: | Alpaca monoclonal Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation), primary antibodies |
|---|---|
| Applications: | WB, ICC/IF, ELISA, IP |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃, (Avoid freeze / thaw cycles), Stable for 12 months at -20℃ |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation) |
| Immunogen: | DYKDDDDK tag fusion protein |
| Recommended Dilutions:
ELISA WB ICC/IF IP Flow Cyt |
1:5,000 -1:20,000 1:5,000 -1:20,000 1:200-1:1,000 2ug/sample 1:200-1:1,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
|
Fig1:
Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (HA710084) at 1/10,000 dilution. Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710084) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot-Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(HA710084) Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(HA710084) at 0.1 µg/ml Lane1: N-terminal DYKDDDDK fusion protein Lane2: C-terminal DYKDDDDK fusion protein Secondary antibody： Anti-Mouse IgG-HRP(HA710142)(1:10000) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.