MBP, AlpHcAbs® Rabbit antibody(Biotin) Alpaca Monoclonal Antibody
cat.: HA710086
Product Type: Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc, primary antibodies
Applications: WB, ELISA, IP
Clonality: Monoclonal
Storage condition: Store at –20 ℃(Avoid freeze / thaw cycles), protect from light,
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Immunogen: MBP fusion protein
Recommended Dilutions:
  WB
  ELISA
  IP

1:5,000-1:20000
1:5,000-1:20000
1:100-1:500
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710086_1.jpg Fig1: Advantages of using an Alpaca Recombinant Secondary
1. Smaller size
Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
2. Easier to use
Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
3. Easier production
When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
4. More consistent
Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
5. Higher Affinity
Nanobodies benefit from consistently high binding affinity values.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.