HRP Conjugated HA tag, AlpHcAbs® Rabbit antibody(HRP) Alpaca Monoclonal Antibody
cat.: HA710114
| Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc, primary antibodies |
|---|---|
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles), |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc |
| Immunogen: | HA tag fused KLH |
| Recommended Dilutions:
WB ELISA |
1:5,000-1:20000 1:5,000-1:20000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA710114) at 1/5,000 dilution. Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Lane 2: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate Lane 3: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate Lane 4: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710114) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig2:
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.