HA tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal
cat.: HA710116
| Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc, primary antibodies |
|---|---|
| Applications: | WB, ICC/IF, ELISA, IP |
| Clonality: | Monoclonal |
| Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles) |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc |
| Immunogen: | HA tag fused KLH |
| Recommended Dilutions:
WB ELISA IP |
1:5,000-1:20000 1:5,000-1:20000 1-2ug/sample, Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA710116) at 1/5,000 dilution. Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Lane 2: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate Lane 3: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate Lane 4: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710116) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot-Anti-HA tag, AlpHcAbs® Mouse antibody(HA710116) Anti-HA tag, AlpHcAbs® Mouse antibody(HA710116) at 0.1 µg/ml Lane1: N-terminal HA tag fusion protein Lane2: C-terminal HA tag fusion protein Secondary antibody： Anti-Mouse IgG-HRP(HA710142)(1:10000) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.