Myc tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal
cat.: HA710120
| Product Type: | Alpaca monoclonal Fab of alpaca IgG1 fused to Rabbit Fc(mutation), primary antibodies |
|---|---|
| Applications: | WB, ICC/IF, ELISA, IP |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃, (Avoid freeze / thaw cycles), Stable for 12 months at -20℃ |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | Fab of alpaca IgG1 fused to Rabbit Fc(mutation) |
| Immunogen: | Myc tag fusion protein |
| Recommended Dilutions:
WB ELISA ICC/IF Flow Cyt |
1:10,000-1:50,000 1:10,000-1:50,000 1:200-1:1000IP: 2ug/sample 1:200-1:2000, Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution. Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat) Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710051) at 1/10,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution. Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat) Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710049) at 1/10,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot-Anti-Myc tag, AlpHcAbs® Rabbit antibody(HA710120) Anti-Myc tag, AlpHcAbs® Rabbit antibody(HA710120) at 0.1 µg/ml Lane1: N-terminal Myc tag fusion protein Lane2: C-terminal Myc tag fusion protein Secondary antibody： Anti-Rabbit IgG-HRP(HA710046)(1:10000) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.