Biotin Conjugated Mouse IgG kappa, AlpHcAbs® Goat antibody(Biotin) Alpaca pacous Monoclonal Antibody
cat.: HA710128
| Product Type: | Alpaca monoclonal, secondary antibodies |
|---|---|
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to goat IgG Fc |
| Immunogen: | Fab region of Mouse IgG |
| Recommended Dilutions:
ELISA WB |
1:5000-1:20000 1:5000-1:20000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
ELISA of specificity for different species of IgG-Anti-Mouse IgG kappa, AlpHcAbs® Goat antibody(Biotin) ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG kappa, AlpHcAbs® Goat antibody(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody. |
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Fig2:
A titer ELISA of mouse IgG-Anti-Mouse IgG kappa, AlpHcAbs® Goat antibody(Biotin) A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG(kappa light chain). 1:5000 dilution of Anti-Mouse IgG kappa, AlpHcAbs® Goat antibody(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.