HRP Conjugated Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) Alpaca pacous Monoclonal Antibody
cat.: HA710154
Product Type: Alpaca monoclonal, secondary antibodies
Applications: WB, ELISA
Clonality: Monoclonal
Storage condition: Store at –20 °C(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20°C
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Isotype: VHH domain of alpaca IgG2b/2c
Immunogen: Recombinant Fc region of mouse IgG2a
Recommended Dilutions:
  ELISA
  WB

1:10000-1:50000
1:10000-1:50000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710154_1.jpg Fig1: ELISA of specificity for different species of IgG-Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
HA710154_2.jpg Fig2: A titer ELISA of mouse IgG2a-Anti-Mouse IgG2a(Fcγ fragment specific), AlpSdAbs® VHH(HRP)
A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. 1:10000 dilution of Anti-Mouse IgG2a(Fcγ fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.