Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody
cat.: HA710156
Product Type: Alpaca monoclonal VHH domain of alpaca IgG2b/2c, secondary antibodies
Applications: WB, ELISA
Clonality: Monoclonal
Storage condition: Store at –20 ℃(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20℃
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Isotype: VHH domain of alpaca IgG2b/2c
Immunogen: Recombinant mouse IgG
Recommended Dilutions:
  ELISA
  WB

1:10,000-1:50,000
1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710156_1.jpg Fig1: ELISA of specificity for different species of IgG-Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
HA710156_2.jpg Fig2: A titer ELISA of mouse IgG-Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP)
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG. 1:10000 dilution of Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
HA710156_3.jpg Fig3: Western blot analysis of Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) on C-FLAG protein with Mouse anti-FLAG antibody (HA601167) at 1/2,000 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 1 minute 7 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601167) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Mouse IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710156) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.