Mouse IgG(H+L), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody
cat.: HA710158
| Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c, secondary antibodies |
|---|---|
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles), Stable for 12 months at -20℃ |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c |
| Immunogen: | Recombinant mouse IgG |
| Recommended Dilutions:
ELISA WB |
1:10,000-1:50,000 1:10,000-1:50,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
ELISA of specificity for different species of IgG-Anti-Mouse IgG(H+L), AlpSdAbs® VHH(HRP) ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG(H+L), AlpSdAbs® VHH(HRP) was used as the detection antibody. |
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Fig2:
A titer ELISA of mouse IgG-Anti-Mouse IgG(H+L), AlpSdAbs® VHH(HRP) A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG. 1:10000 dilution of Anti-Mouse IgG(H+L), AlpSdAbs® VHH(HRP) was used as the detection antibody. |
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Fig3:
Western blot analysis of Mouse IgG(H+L), AlpSdAbs® VHH(HRP) on C-FLAG protein with Mouse anti-FLAG antibody (HA601167) at 1/2,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601167) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Mouse IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710158) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.