Product Type: | Alpaca monoclonal, secondary antibodies |
---|---|
Applications: | WB, ELISA |
Clonality: | Monoclonal |
Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20°C |
Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol |
Concentration: | 1ug/ul |
Purification: | Recombinant Expression and Affinity purified |
Isotype: | VHH domain of alpaca IgG2b/2c |
Immunogen: | Recombinant mouse IgG |
Recommended Dilutions:
ELISA WB |
1:10000-1:50000 1:10000-1:50000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Fig1:
ELISA of specificity for different species of IgG-Anti-Mouse IgG(H+L), AlpSdAbs® VHH ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG(H+L), AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG(HA710110) as the secondary antibody. |
|
Fig2:
A titer ELISA of mouse IgG-Anti-Mouse IgG(H+L), AlpSdAbs® VHH A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG. 1:10000 dilution of Anti-Mouse IgG(H+L), AlpSdAbs® VHH was used as the primary antibody. An HRP conjugated Anti-His Rabbit IgG(HA710110) as the secondary antibody. |