Product Type: | Alpaca monoclonal, secondary antibodies |
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Applications: | WB |
Clonality: | Monoclonal |
Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), protect from light |
Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol |
Concentration: | 1ug/ul |
Purification: | Recombinant Expression and Affinity purified |
Isotype: | VHH domain of alpaca IgG2b/2c |
Immunogen: | Mouse IgG |
Recommended Dilutions:
WB |
1:5,000-1:10,000, Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Fig1:
Western blot-Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)(HA710160) Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)(HA710160) at 0.1 µg/ml Lane 1 : Mouse IgG1(non-reduced) Lane 2 : Mouse IgG1(reduced) Lane 3 : Mouse IgG2a(non-reduced) Lane 4 : Mouse IgG2a(reduced) Lane 5 : Mouse IgG2b(non-reduced) Lane 6 : Mouse IgG2b(reduced) Lane 7 : Mouse IgG3(non-reduced) Lane 8 : Mouse IgG3(reduced) Mouse IgG at 0.2 µg per lane. |