Biotin Conjugated Goat IgG(H+L), AlpSdAbs® VHH(Biotin) Alpaca pacous Monoclonal Antibody
cat.: HA710177
Product Type: Alpaca monoclonal, secondary antibodies
Applications: WB, ELISA, IP
Clonality: Monoclonal
Form: Liquid
Storage condition: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Storage buffer: 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Immunogen: Recombinant goat IgG
Recommended Dilutions:
  ELISA
  WB
  IP

1:5000-1:20000
1:5000-1:20000
1-2ug/sampleBLI (biolayer interferometry)SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710177_1.jpg Fig1: Advantages of using an Alpaca Recombinant Secondary
1. Smaller size
Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
2. Easier to use
Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
3. Easier production
When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
4. More consistent
Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
5. Higher Affinity
Nanobodies benefit from consistently high binding affinity values.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.