Biotin Conjugated Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) Alpaca pacous Monoclonal Antibody
cat.: HA710194
Product Type: Alpaca monoclonal, secondary antibodies
Applications: ELISA, Purification
Clonality: Monoclonal
Storage condition: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Concentration: 1ug/ul
Purification: Recombinant Expression and Affinity purified
Immunogen: Recombinant Fc region of mouse IgG1
Recommended Dilutions:
  ELISA
  WB
  IP

1:10000-1:50000
1:10000-1:50000
1-2ug/sample,BLI (biolayer interferometry),SPR (surface plasmon resonance),
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Images
HA710194_1.jpg Fig1: ELISA of specificity for different species of IgG-Anti-Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody.
HA710194_2.jpg Fig2: A titer ELISA of mouse IgG1-Anti-Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin)
A titer ELISA of mouse IgG1. The plate was coated with different amounts of mouse IgG1. 1:10000 dilution of Anti-Mouse IgG1(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.