Biotin Conjugated Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) Alpaca pacous Monoclonal Antibody
cat.: HA710198
| Product Type: | Alpaca monoclonal, secondary antibodies |
|---|---|
| Applications: | ELISA, Purification |
| Clonality: | Monoclonal |
| Storage condition: | Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C |
| Storage buffer: | 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Immunogen: | Recombinant Fc region of mouse IgG2b |
| Recommended Dilutions:
ELISA WB IP |
1:10000-1:50000 1:10000-1:50000 1-2ug/sample,BLI (biolayer interferometry),SPR (surface plasmon resonance), Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
ELISA of specificity for different species of IgG-Anti-Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody. |
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Fig2:
A titer ELISA of mouse IgG2b-Anti-Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. 1:10000 dilution of Anti-Mouse IgG2b(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) was used as the primary antibody. An HRP conjugated streptavidin as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.