iFluor™ 647 Conjugated HA tag, AlpHcAbs® Mouse IgG2a Alpaca Monoclonal Antibody
cat.: HA710217
| Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to mouse IgG2a Fc(mutation), primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ICC/IF, ELISA, FC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃ (Avoid freeze / thaw cycles), protect from light |
| Storage buffer: | Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to mouse IgG2a Fc(mutation) |
| Immunogen: | HA tag fused KLH |
| Recommended Dilutions:
WB ICC/IF ELISA FC |
1:5000-1:20000 1:200-1:1000 1:5000-1:2000 1µg for 106 cells Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Images
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Fig1:
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.