Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation), secondary antibodies |
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Species reactivity: | Rat |
Applications: | ELISA, WB, ICC/IF, IP, FC |
Clonality: | Monoclonal |
Form: | Liquid |
Storage condition: | Store at –20 ℃(Avoid freeze / thaw cycles) |
Storage buffer: | Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Isotype: | VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) |
Immunogen: | Rat IgG Fab |
Recommended Dilutions:
ELISA WB ICC/IF IP FC |
1:5,000-1:20,000 1:5,000-1:20,000 1:200-1:1,000 1-2ug/sample 1µg for 10^6 cells |
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Fig1:
Advantages of using an Alpaca Recombinant Secondary 1. Smaller size Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies. 2. Easier to use Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. 3. Easier production When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. 4. More consistent Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. 5. Higher Affinity Nanobodies benefit from consistently high binding affinity values. |