StayGold, AlpHcAbs® Rabbit Alpaca Monoclonal Antibody [A2F7]
cat.: HA710349
| Product Type: | Alpaca monoclonal VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation), primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, ICC/IF, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | A2F7 |
| Form: | Liquid |
| Storage condition: | Store at –20 ℃, (Avoid freeze / thaw cycles) |
| Storage buffer: | Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol |
| Concentration: | 1ug/ul |
| Purification: | Recombinant Expression and Affinity purified |
| Isotype: | VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation) |
| Immunogen: | StayGold fusion protein |
| Recommended Dilutions:
WB ELISA ICC/IF IP FC |
1:5,000-1:20,000 1:5,000-1:20,000 1:100-1:1,000 1-2ug/sample 1µg for 10^6 cells |
Images
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Fig1:
Lane1-6: different amount(200ng-100ng-50ng-25ng-12.5-6.25ng) of mBaojin was loaded. First antibody:Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(HA710349) at 0.1 µg/ml (1:10000) Secondary antibody： Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody (HRP) (1:10000) |
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Fig2: The plate was coated with different amounts of StayGold or its variants. 1:10000 dilution of Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody was used as the primary antibody. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH (HRP) was used as the secondary antibody. |
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Fig3: StayGold-Ensconsin transfected COS-7 cells were stained with Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody (1:500) and Goat Anti-Rabbit IgG Antibody(AF647) was the secondary antibody(1:1000). (This picture is kindly provided by Zhang Lab, School of Life Sciences, Westlake University) |
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Fig4: Different amount of reduced StayGold is detected by Anti-StayGold, AlpHcAbs® Rabbit antibody with 1:5000 dilution, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH (HRP) is used as the secondary antibody. |
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Fig5: The plate was coated with different amounts of StayGold or its variants. 1:5000 dilution of Anti-StayGold, AlpHcAbs® Rabbit antibody was used as the detection antibody, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH (HRP) is used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.