SLC27A4 Recombinant Rabbit Monoclonal Antibody [JE58-29]
cat.: HA720001
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE58-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 72 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SLC27A4 aa 393-643.
Positive control: Mouse pancreas tissue lysates, rat kidney tissue, mouse small intestine tissue, human rectal cancer tissue, A549.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q6P1M0 Human | Q91VE0 Mouse
Alternative names: ACSVL 4 ACSVL4 EC 6.2.1 FATP 4 FATP4 Fatty acid transport protein 4 Fatty acid transport protein4 IPS Long chain fatty acid transport protein 4 Long chain fatty acid transport protein4 OTTHUMP00000022264 S27A4 SLC27 A4 SLC27A 4 Solute carrier family 27 (fatty acid transporter) member 4 Solute carrier family 27 member 4 Solute carrier family 27 member4
Images
HA720001_1.jpg Fig1: Western blot analysis of SLC27A4 on different lysates with Rabbit anti-SLC27A4 antibody (HA720001) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-SLC27A4 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 60 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720001) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720001_2.jpg Fig2: Western blot analysis of SLC27A4 on mouse pancreas tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720001, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HA720001_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SLC27A4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720001, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720001_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SLC27A4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720001, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720001_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using anti-SLC27A4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720001, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720001_6.jpg Fig6: Flow cytometric analysis of SLC27A4 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720001, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.