| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IGF2BP2 aa 100-200. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, rat bone marrow tissue lysates. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9Y6M1 Human |
| Alternative names: | Hepatocellular carcinoma autoantigen p62 IF2B2_HUMAN IGF 2 mRNA binding protein 2 IGF II mRNA binding protein 2 IGF-II mRNA-binding protein 2 IGF2 mRNA-binding protein 2 Igf2bp2 IMP 2 IMP-2 IMP2 Insulin like growth factor 2 mRNA binding protein 2 Insulin-like growth factor 2 mRNA-binding protein 2 OTTHUMP00000082606 OTTHUMP00000082607 OTTHUMP00000082608 p62 VICKZ 2 VICKZ family member 2 VICKZ2 |
|
Fig1:
Western blot analysis of IGF2BP2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate |
|
Fig2: Western blot analysis of IGF2BP2 on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |