Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE56-93 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 21 kDa. |
Isotype: | IgG |
Immunogen: | Recombinant protein within human SLC31A1 aa 1-82. |
Positive control: | SW480 cell lysate, A549 cell lysate, HepG2 cell lysate, SKOV-3 cell lysate, 293 cell lysate, HCT116 cell lysate, rat testis tissue lysate, mouse spleen lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: O15431 Human | Q8K211 Mouse | Q9JK41 Rat |
Alternative names: | Copper transport 1 homolog Copper transporter 1 COPT1 COPT1_HUMAN CTR1 hCTR1 High affinity copper uptake protein 1 SLC31A1 solute carrier family 31 (copper tansporters) member 1 Solute carrier family 31 member 1 |
Fig1:
Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: SKOV-3 cell lysate Lane 5: 293 cell lysate Lane 6: HCT116 cell lysate |
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Fig2:
Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat testis tissue lysate Lane 2: Mouse spleen lysate |