ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67]
cat.: HA720006
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ACAA2 aa 347-397. |
| Positive control: | 293T cell lysate, rat colon tissue lysate, NIH/3T3 cell lysate, rat kidney tissue, human liver tissue, human appendix tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P42765 Human | Q8BWT1 Mouse | P13437 Rat |
| Alternative names: | 3 ketoacyl CoA thiolase 3 ketoacyl CoA thiolase mitochondrial 3-ketoacyl-CoA thiolase, mitochondrial 3-oxoacyl-CoA thiolase, mitochondrial ACAA 2 Acaa2 Acetyl CoA acyltransferase 2 Acetyl CoA acyltransferase Acetyl Coenzyme A acyltransferase 2 Acetyl-CoA acyltransferase Beta ketothiolase Beta-ketothiolase DSAEC Mitochondrial 3 oxoacyl CoA thiolase Mitochondrial 3 oxoacyl Coenzyme A thiolase Mitochondrial 3-oxoacyl-CoA thiolase T1 THIM_HUMAN |
Images
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Fig1:
Western blot analysis of ACAA2 on different lysates with Rabbit anti-ACAA2 antibody (HA720006) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACAA2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720006) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ACAA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720006, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Rat colon tissue lysate Lane 3: NIH/3T3 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.