Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]
cat.: HA720008
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE56-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Sulfite oxidase aa 100-200. |
| Positive control: | 293T cell lysate, Mouse liver tissue lysate, human kidney tissue, HepG2. |
| Subcellular location: | Mitochondrion intermembrane space. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: P51687 Human | Q8R086 Mouse |
| Alternative names: | EC 1.8.3.1 mitochondrial Sulfite oxidase Sulfite oxidase mitochondrial Sulfite oxidase, mitochondrial precursor Suox SUOX_HUMAN |
Images
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Fig1:
Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Sulfite oxidase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Sulfite oxidase was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720008, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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