Erlin-2 Recombinant Rabbit Monoclonal Antibody [JE57-03]
cat.: HA720009
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE57-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human Erlin-2 aa 50-200 (Lumenal).
Positive control: Mouse kidney tissue lysates, SKOV-3 cell lysates, human colon carcinoma tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: O94905 Human | Q8BFZ9 Mouse
Alternative names: C8orf2 Chromosome 8 open reading frame 2 Endoplasmic reticulum lipid raft associated protein 2 Endoplasmic reticulum lipid raft-associated protein 2 ER lipid raft associated 2 ERLIN 2 Erlin-2 ERLIN2 ERLN2_HUMAN HGNC:1356 MGC87072 NET32 SPFH 2 SPFH domain containing protein 2 SPFH domain family member 2 SPFH domain-containing protein 2 SPFH2 SPG18 Stomatin prohibitin flotillin HflC/K domain containing protein 2 Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
Images
HA720009_1.jpg Fig1: Western blot analysis of Erlin-2 on different lysates with Rabbit anti-Erlin-2 antibody (HA720009) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD Erlin-2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 40 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720009) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720009_2.jpg Fig2: Western blot analysis of Erlin-2 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720009, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA720009_3.jpg Fig3: Western blot analysis of Erlin-2 on SKOV-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720009, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA720009_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720009, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720009_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720009, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720009_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720009, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720009_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720009, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.