WIPI1 Recombinant Rabbit Monoclonal Antibody [JE56-92]
cat.: HA720010
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WIPI1 aa 20-120. |
| Positive control: | JAR cell lysates, human fetal skeletal muscle tissue. |
| Subcellular location: | Endosome, trans-Golgi network, cytoskeleton, clathrin-coated vesicle, preautophagosomal structure membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q5MNZ9 Human |
| Alternative names: | ATG 18 ATG18 Atg18 protein homolog ATG18A FLJ10055 WD repeat domain phosphoinositide interacting 1 WD repeat domain phosphoinositide interacting protein 1 WD repeat domain phosphoinositide-interacting protein 1 WD40 repeat protein interacting with phosphoinositides of 49 kDa WD40 repeat protein interacting with phosphoInositides of 49kDa WIPI 1 alpha WIPI 1 WIPI 49 WIPI 49 kDa WIPI-1 wipi1 WIPI1_HUMAN WIPI49 |
Images
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Fig1: Western blot analysis of WIPI1 on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-WIPI1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720010, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.