Human Kappa light chain Recombinant Rabbit Monoclonal Antibody [JE56-54]
cat.: HA720014
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12/13 kDa |
| Isotype: | IgG |
| Immunogen: | Full length native human IgG. |
| Positive control: | Human plasma lysates, human spleen tissue lysate, human thymus tissue lysate, human plasma tissue lysate, human tonsil tissue, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P01601 Human | P01834 Human |
| Alternative names: | Immunoglobulin kappa variable 1D-16 Ig kappa chain V-I region HK146 Ig kappa chain V-I region HK189 Immunoglobulin kappa constant Ig kappa chain C regionCurated Ig kappa chain C region AG Ig kappa chain C region CUM Ig kappa chain C region EU Ig kappa chain C region OU Ig kappa chain C region ROY Ig kappa chain C region TI |
Images
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Fig1:
Western blot analysis of Human Kappa light chain on human plasma lysates with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 12/13 kDa Observed band size: 25 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720014) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Human Kappa light chain on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720014, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human spleen tissue lysate Lane 2: Human thymus tissue lysate Lane 3: Human plasma tissue lysate |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Human Kappa light chain antibody (HA720014) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.