NUDT5 Recombinant Rabbit Monoclonal Antibody [JE56-41]
cat.: HA720015
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NUDT5 aa 1-150. |
| Positive control: | Hela cell lysate, 293 cell lysate, human liver tissue, human liver carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UKK9 Human |
| Alternative names: | ADP sugar pyrophosphatase ADP-sugar pyrophosphatase hYSAH 1 hYSAH1 Nucleoside diphosphate linked moiety X motif 5 Nucleoside diphosphate linked moiety X type motif 5 Nucleoside diphosphate-linked moiety X motif 5 Nudix (nucleoside diphosphate linked moiety X) type motif 5 Nudix motif 5 Nudix type motif 5 NUDT 5 Nudt5 NUDT5_HUMAN YSA1 YSA1H |
Images
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Fig1:
Western blot analysis of NUDT5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720015, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NUDT5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720015, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-NUDT5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720015, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.