Olig3 Recombinant Rabbit Monoclonal Antibody [JE56-40]
cat.: HA720016
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE56-40
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human Olig3 aa 222-272.
Positive control: Human placenta tissue lysates, F9, human kidney tissue, human esophagus tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: Q7RTU3 Human | Q6PFG8 Mouse
Alternative names: "Basic helix loop helix domain containing class B protein 7 Basic helix-loop-helix domain-containing protein, class B, 7 bHLHB7 bHLHe20 Class B basic helix loop helix protein 7 Class B basic helix-loop-helix protein 7 Class E basic helix loop helix protein 20 Class E basic helix-loop-helix protein 20 Olig3 OLIG3_HUMAN Oligo3 Oligodendrocyte lineage transcription factor 3 Oligodendrocyte specific bHLH transcription factor 3 Oligodendrocyte transcription factor 3 "
Images
HA720016_1.jpg Fig1: Western blot analysis of Olig3 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720016, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720016_2.jpg Fig2: ICC staining of Olig3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720016, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA720016_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Olig3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720016, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720016_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Olig3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720016, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.