NCS1 Recombinant Rabbit Monoclonal Antibody [JE56-39]
cat.: HA720017
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NCS1 aa 90-190. |
| Positive control: | SH-SY5Y cell lysate, A549 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus, postsynaptic density, perinuclear region, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P62166 Human | Q8BNY6 Mouse | P62168 Rat |
| Alternative names: | 9430075O15Rik A730032G13Rik AI836659 DKFZp761L1223 FLUP FREQ Frequenin Frequenin homolog (Drosophila) Frequenin homolog Frequenin like protein Frequenin, Drosophila, homolog of Frequenin-like protein Frequenin-like ubiquitous protein Mfreq NCS 1 NCS-1 ncs1 NCS1_HUMAN Neuronal calcium sensor 1 Neuronal Calicum Sensor 1 |
Images
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Fig1:
Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (HA720017) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NCS1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720017) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (HA720017) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A549 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720017) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.