Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE56-39 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 22 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human NCS1 aa 90-190. |
Positive control: | SH-SY5Y cell lysate, rat brain tissue lysate, mouse cerebellum tissue lysate. |
Subcellular location: | Cell membrane, Golgi apparatus, postsynaptic density, perinuclear region, Cytoplasm, Membrane. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: P62166 Human | Q8BNY6 Mouse | P62168 Rat |
Alternative names: | 9430075O15Rik A730032G13Rik AI836659 DKFZp761L1223 FLUP FREQ Frequenin Frequenin homolog (Drosophila) Frequenin homolog Frequenin like protein Frequenin, Drosophila, homolog of Frequenin-like protein Frequenin-like ubiquitous protein Mfreq NCS 1 NCS-1 ncs1 NCS1_HUMAN Neuronal calcium sensor 1 Neuronal Calicum Sensor 1 |
Fig1:
Western blot analysis of NCS1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720017, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse cerebellum tissue lysate |