Uridine Phosphorylase 1 Recombinant Rabbit Monoclonal Antibody [JE56-62]
cat.: HA720018
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE56-62
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human Uridine Phosphorylase 1 aa 1-50.
Positive control: PANC-1 cell lysate,AsPC-1 cell lysate , F9 cell lysate
Subcellular location: Cytosol, nucleoplasm.
Recommended Dilutions:
  WB

1:500-1:5,000
Uniprot #: SwissProt: Q16831 Human | P52624 Mouse
Alternative names: UDRPASE UP UPase 1 UPASE UPP Upp1 UPP1_HUMAN UrdPase 1 Uridine phosphorylase 1
Images
HA720018_1.jpg Fig1: Western blot analysis of Uridine Phosphorylase 1 on different lysates with Rabbit anti-Uridine Phosphorylase 1 antibody (HA720018) at 1/5,000 dilution.

Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 2: AsPC-1 (Human pancreatic cancer cell) cell lysate
Lane 3: F9 (Mouse teratocarcinoma cell)

Lysates/proteins at 15 µg/Lane
Exposure time: 62 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720018, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 33 kDa
Observed band size: 33 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.