| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Uridine Phosphorylase 1 aa 1-50. |
| Positive control: | A431 cell lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Cytosol, nucleoplasm. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q16831 Human | P52624 Mouse |
| Alternative names: | UDRPASE UP UPase 1 UPASE UPP Upp1 UPP1_HUMAN UrdPase 1 Uridine phosphorylase 1 |
|
Fig1:
Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat lung tissue lysate Lane 4: Rat kidney tissue lysate |