| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Uridine Phosphorylase 1 aa 1-50. |
| Positive control: | PANC-1 cell lysate,AsPC-1 cell lysate , F9 cell lysate |
| Subcellular location: | Cytosol, nucleoplasm. |
| Recommended Dilutions:
WB |
1:500-1:5,000 |
| Uniprot #: | SwissProt: Q16831 Human | P52624 Mouse |
| Alternative names: | UDRPASE UP UPase 1 UPASE UPP Upp1 UPP1_HUMAN UrdPase 1 Uridine phosphorylase 1 |
|
Fig1:
Western blot analysis of Uridine Phosphorylase 1 on different lysates with Rabbit anti-Uridine Phosphorylase 1 antibody (HA720018) at 1/5,000 dilution. Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 2: AsPC-1 (Human pancreatic cancer cell) cell lysate Lane 3: F9 (Mouse teratocarcinoma cell) Lysates/proteins at 15 µg/Lane Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720018, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 33 kDa |