| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CRMP3 aa 1-100. |
| Positive control: | SH-SY5Y cell lysates, rat cerebellum tissue lysate,mouse cerebellum tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: O14531 Human | O35098 Mouse | Q62951 Rat |
| Alternative names: | Collapsin response mediator protein 3 CRMP 3 CRMP-3 CRMP3 Dihydropyrimidinase like 4 Dihydropyrimidinase related protein 4 Dihydropyrimidinase-related protein 4 DPYL4_HUMAN DPYSL4 DRP 4 DRP-4 DRP4 ULIP 4 ULIP-4 ULIP4 UNC33 like phosphoprotein 4 UNC33-like phosphoprotein 4 |
|
Fig1:
Western blot analysis of CRMP3 on different lysates with Rabbit anti-CRMP3 antibody (HA720021) at 1/10,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720021, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 62 kDa Observed band size: 62 kDa |