Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE56-59 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 62 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human CRMP3 aa 1-100. |
Positive control: | SH-SY5Y cell lysates, rat brain tissue lysate, mouse hippocampus tissue lysate. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: O14531 Human | O35098 Mouse | Q62951 Rat |
Alternative names: | Collapsin response mediator protein 3 CRMP 3 CRMP-3 CRMP3 Dihydropyrimidinase like 4 Dihydropyrimidinase related protein 4 Dihydropyrimidinase-related protein 4 DPYL4_HUMAN DPYSL4 DRP 4 DRP-4 DRP4 ULIP 4 ULIP-4 ULIP4 UNC33 like phosphoprotein 4 UNC33-like phosphoprotein 4 |
Fig1: Western blot analysis of CRMP3 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720021, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. | |
Fig2:
Western blot analysis of CRMP3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720021, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse hippocampus tissue lysate |