USP24 Recombinant Rabbit Monoclonal Antibody [JE56-50]
cat.: HA720022
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE56-50
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size:294 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human USP24.
Positive control: NIH/3T3 cell lysates, HepG2, human liver carcinoma tissue, human fetal skeletal muscle tissue, mouse liver tissue, mouse small intestine tissue.
Subcellular location: Cytosol, nucleoplasm, nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: Q9UPU5 Human | B1AY13 Mouse
Alternative names: "Deubiquitinating enzyme 24 KIAA1057 Ubiquitin carboxyl terminal hydrolase 24 Ubiquitin carboxyl-terminal hydrolase 24 Ubiquitin specific peptidase 24 Ubiquitin specific processing protease 24 Ubiquitin specific protease 24 Ubiquitin thioesterase 24 Ubiquitin thiolesterase 24 Ubiquitin-specific-processing protease 24 UBP24_HUMAN Usp24 "
Images
HA720022_1.jpg Fig1: Western blot analysis of USP24 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720022, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA720022_2.jpg Fig2: ICC staining of USP24 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720022, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA720022_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-USP24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720022, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720022_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720022, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720022_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-USP24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720022, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720022_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-USP24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720022, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.