USP24 Recombinant Rabbit Monoclonal Antibody [JE56-50]
cat.: HA720022
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE56-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 294 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human USP24. |
| Positive control: | A549-parental cell lysate, A549-商品名 KD cell lysate, HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse lung tissue lysate, HepG2. |
| Subcellular location: | Cytosol, nucleoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UPU5 Human | B1AY13 Mouse |
| Alternative names: | Deubiquitinating enzyme 24 KIAA1057 Ubiquitin carboxyl terminal hydrolase 24 Ubiquitin carboxyl-terminal hydrolase 24 Ubiquitin specific peptidase 24 Ubiquitin specific processing protease 24 Ubiquitin specific protease 24 Ubiquitin thioesterase 24 Ubiquitin thiolesterase 24 Ubiquitin-specific-processing protease 24 UBP24_HUMAN Usp24 |
Images
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Fig1:
Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (HA720022) at 1/1,000 dilution. Lane 1: A549-parental cell lysate Lane 2: A549-USP24 KD cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 294 kDa Observed band size: 294 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (HA720022) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: Jurkat cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 294 kDa Observed band size: 294 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720022) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: ICC staining of USP24 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720022, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.