ALKBH1 Recombinant Rabbit Monoclonal Antibody [JE54-26]
cat.: HA720024
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE54-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human ALKBH1. |
| Positive control: | Human tonsil tissue, Human colon tissue, K-562 cell lysate, HEK-293 cell lysate. |
| Subcellular location: | Nucleus, mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13686 Human |
| Alternative names: | ABH ABH1 alkB alkB homolog 1 histone H2A dioxygenase AlkB, alkylation repair homolog 1 (E. coli) ALKB1_HUMAN ALKBH ALKBH1 Alkylated DNA repair protein alkB homolog 1 Alkylation repair homolog 1 Alkylation repair, alkB homolog Alpha-ketoglutarate-dependent dioxygenase ABH1 DNA lyase ABH1 DNA oxidative demethylase ALKBH1 hABH |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720024, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded Human colon tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720024, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of ALKBH1 on different lysates with Rabbit anti-ALKBH1 antibody (HA720024) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA720024, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size:44 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.