CPSF73 Recombinant Rabbit Monoclonal Antibody [JE59-11]
cat.: HA720025
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE59-11
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CPSF73 aa 585-684/684.
Positive control: 293T cell lysates, rat large intestine tissue, human liver carcinoma tissue, human kidney tissue, human fallopian tube tissue, human small intestine tissue, mouse cerebellum tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q9UKF6 Human | Q9QXK7 Mouse
Alternative names: Cleavage and polyadenylation specific factor 3 73kDa Cleavage and polyadenylation specificity factor 73 kDa subunit Cleavage and polyadenylation specificity factor subunit 3 CPSF 73 CPSF 73 kDa subunit CPSF CPSF-73 cpsf3 CPSF3_HUMAN CPSF73 mRNA 3''-end-processing endonuclease CPSF-73 YSH1
Images
HA720025_1.jpg Fig1: Western blot analysis of CPSF73 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA720025_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720025_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720025_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720025_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720025_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720025_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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