ZPR1 Recombinant Rabbit Monoclonal Antibody [JE58-88]
cat.: HA720026
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE58-88
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ZPR1 aa 360-459/459.
Positive control: HCT 116 cell lysate, HepG2 cell lysate, MDA-MB-231 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, MEF cell lysate, PC-12 cell lysate, PC-12, human testis tissue, mouse testis tissue, rat testis tissue, HCT 116, NIH/3T3.
Subcellular location: Nucleus, nucleolus, gem, Cajal body, perinuclear region, Cytoplasm, axon, growth cone.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:5,000
1:50-1:200
1:100
1:1,000
Uniprot #: SwissProt: O75312 Human | Q62384 Mouse
Entrez Gene: 500989 Rat
Alternative names: MGC110983 Zinc finger protein 259 Zinc finger protein ZPR1 ZNF 259 ZNF259 ZPR1 ZPR1_HUMAN
Images
HA720026_1.jpg Fig1: Western blot analysis of ZPR1 on different lysates with Rabbit anti-ZPR1 antibody (HA720026) at 1/5,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: MEF cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 8 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720026) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720026_2.jpg Fig2: Immunocytochemistry analysis of PC-12 cells labeling ZPR1 with Rabbit anti-ZPR1 antibody (HA720026) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZPR1 antibody (HA720026) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA720026_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ZPR1 antibody (HA720026) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720026) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720026_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ZPR1 antibody (HA720026) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720026) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720026_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ZPR1 antibody (HA720026) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720026) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720026_6.jpg Fig6: Flow cytometric analysis of HCT 116 cells labeling ZPR1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA720026, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA720026_7.jpg Fig7: Flow cytometric analysis of NIH/3T3 cells labeling ZPR1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA720026, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA720026_8.jpg Fig8: Flow cytometric analysis of PC-12 cells labeling ZPR1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA720026, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.