RCL Recombinant Rabbit Monoclonal Antibody [JE59-29]
cat.: HA720027
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE59-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RCL aa 75-174/174.
Positive control: K562 cell lysate, Jurkat cell lysate, 293T cell lysate, HepG2 cell lysate, human liver tissue, human kidney tissue, A431, THP-1.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2,000
1:100-1:200
1:50-1:200
1:500-1:1,000
Uniprot #: SwissProt: O43598 Human
Alternative names: 2' deoxynucleoside 5' phosphate N hydrolase 1 BC048355 c Myc responsive c Myc responsive protein Rcl c-Myc-responsive protein Rcl C6orf108 C76683 Chromosome 6 open reading frame 108 Deoxyribonucleoside 5' monophosphate N glycosidase Deoxyribonucleoside 5''-monophosphate N-glycosidase dJ330M21.3 DNPH1 MGC54855 Putative c Myc responsive Rcl RCL_HUMAN RP3 330M21.3
Images
HA720027_1.jpg Fig1: Western blot analysis of RCL on different lysates with Rabbit anti-RCL antibody (HA720027) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RCL KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 180 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720027) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA720027_2.jpg Fig2: Western blot analysis of RCL on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720027, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: HepG2 cell lysate
HA720027_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RCL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720027, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720027_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RCL antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720027, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA720027_5.jpg Fig5: ICC staining of RCL in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720027, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA720027_6.jpg Fig6: Flow cytometric analysis of RCL was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720027, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.